Vibriophage D10 contains non-permuted DNA with cohesive ends
نویسندگان
چکیده
منابع مشابه
Structure of vibriophage D10 tail sheath revealed by electron microscopy and computational image processing.
Vibriophage D10, a member of the Vibrio cholerae O-1El-Tor phage typing scheme, is used to detect the spread of the cholera epidemic and belongs to the Myoviridae family. The outer sheath of the tail of vibriophage is highly contractile in nature. We have used electron microscopy and computational image-processing techniques to determine the structure of this contractile tail sheath. The three-...
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Procedures have been worked out for Aspergillus nuclease S1 and mung been nuclease to quantitatively cleave off both of the 12-nucleotide long, single-stranded cohesive ends of lambdaDNA. This cleavage is indicated by the almost complete elimination of the repair incorporation of radioactive nucleotides by DNA polymerase into the digested DNA. With S1 nuclease, cleavage was complete at 10 degre...
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The p53 gene regulates the cell cycle response to DNA damage, which may allow time for adequate DNA repair. We asked whether p53 could directly increase the repair of defined double-strand breaks (DSBs) by nonhomologous end-joining in gamma-irradiated mouse embryonic fibroblasts with differing p53 status. By using an episomal plasmid reactivation assay, we found that presence of wild-type p53 e...
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The p53 gene regulates the cell cycle response to DNA damage, which may allow time for adequate DNA repair. We asked whether p53 could directly increase the repair of defined double-strand breaks (DSBs) by nonhomologous end-joining in g-irradiated mouse embryonic fibroblasts with differing p53 status. By using an episomal plasmid reactivation assay, we found that presence of wild-type p53 enhan...
متن کاملVibriophage and Cholera Disease
Phage typing is an important tool to discriminate the strains of Vibrio cholerae from epidemiological point of view. National Institute of Cholera & Enteric Diseases is engaged in research on cholera phages since decades in India. Bacteriophages have long been used for classification and discrimination of bacterial strains. Cholera, an endemic disease in Indian subcontinent with mild to lethal ...
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ژورنال
عنوان ژورنال: Journal of General Virology
سال: 1993
ISSN: 0022-1317,1465-2099
DOI: 10.1099/0022-1317-74-12-2749